Simultaneous COD Removal and PHA Production in an Activated Sludge System under Different Temperatures
Keywords:Activated sludge, polyhydroxyalkanoate, nutrient limitation, biodegradable plastics, temperature effects.
The purpose of this study was to investigate PHA production by activated sludge biomass under different temperatures. The two-step approach, i.e. utilizing a growth phase followed by a nutrient(s) limitation phase, was applied to stimulate PHA accumulation. Each of three intended temperatures, 10oC, 20oC and 30oC, was investigated with combined N&P limitation. Four liter (L) fully aerobic SBRs were used for all experiments, and operated with a 6 h cycle time, a 10 h HRT and a 10 day SRT. The maximum PHA cellular contents and total concentrations achieved during the N&P limitation periods in the 10 and 20oC systems were very similar, i.e. 45 and 43% of the TSS and 2133 and 2239 mg/l, respectively, whereas the 30oC results were lower at 33% of TSS and 1476 mg/l. The biomass temperature clearly had a strong inverse effect upon PHA productivity. It decreased from 427 and 204 to 148 mg/l-day as reactor temperature increased from 10 to 20 and 30oC, respectively. As well, the PHA yields decreased from 0.38 to 0.16 and 0.11 mg PHA/mg CODu, respectively. The results strongly indicate that activated sludge PHA accumulation stimulated by combined N and P limitation is inversely correlated with temperature.
How to Cite
Authors who publish with Engineering Journal agree to transfer all copyright rights in and to the above work to the Engineering Journal (EJ)'s Editorial Board so that EJ's Editorial Board shall have the right to publish the work for nonprofit use in any media or form. In return, authors retain: (1) all proprietary rights other than copyright; (2) re-use of all or part of the above paper in their other work; (3) right to reproduce or authorize others to reproduce the above paper for authors' personal use or for company use if the source and EJ's copyright notice is indicated, and if the reproduction is not made for the purpose of sale.