Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Authors

  • Rintaro Hoshino Nagoya University
  • Kazuya Murakami Nagoya University
  • Wahyudiono Wahyudiono Nagoya University
  • Siti Machmudah Sepuluh Nopember Institute of Technology
  • Yuji Okita Nippon Suisan Kaisha, Ltd.
  • Eiji Ohashi Nippon Suisan Kaisha, Ltd.
  • Hideki Kanda Nagoya University
  • Motonobu Goto Nagoya University

DOI:

https://doi.org/10.4186/ej.2016.20.4.145

Keywords:

Aurantiochytrium limacinum, liquefied dimethyl ether, supercritical carbon dioxide, fatty acid, bio fuel, dewatering.

Abstract

Recently, a simple method for the extraction of lipids from wet biomass using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Here, the versatility of this method was evaluated for labyrinthula Aurantiochytrium limacinum (A. limacinum). The liquefied DME was passed through the extractor that filled by A. limacinum at different time intervals. The extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, the yield of lipid was 46.1 wt% of the dry weight of the sample. In comparison, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% when supercritical carbon dioxide (SCCO2), hexane-Soxhlet and Bligh-Dyer (BD) extraction methods were applied as extractants, respectively. However, the drying and cell-disruption process were required in SCCO2, hexane-Soxhlet, and BD extraction methods.

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Author Biographies

Rintaro Hoshino
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Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan

Kazuya Murakami
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Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan

Wahyudiono Wahyudiono
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Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan

Siti Machmudah
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Department of Chemical Engineering, Sepuluh Nopember Institute of Technology, Kampus ITS Sukolilo, Surabaya 60111, Indonesia

Yuji Okita
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Nippon Suisan Kaisha, Ltd., Hachioji, Tokyo 192-0991, Japan

Eiji Ohashi
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Nippon Suisan Kaisha, Ltd., Hachioji, Tokyo 192-0991, Japan

Hideki Kanda
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Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan
Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan

Motonobu Goto
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Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan

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Published In
Vol 20 No 4, Aug 29, 2016
How to Cite
[1]
R. Hoshino, “Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether”, Eng. J., vol. 20, no. 4, pp. 145-153, Aug. 2016.
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