Effect of Silver Sulfadiazine and Metallic Ions on Properties of Thai Silk Fibroin/Gelatin Films for Anti-Bacterial Applications

Authors

  • Patchara Umprasert Chulalongkorn University
  • Siriporn Damrongsakkul Chulalongkorn University
  • Supansa Yodmuang Chulalongkorn University
  • Juthamas Ratanavaraporn Chulalongkorn University

DOI:

https://doi.org/10.4186/ej.2021.25.5.13

Keywords:

Silk fibroin, gelatin, silver sulfadiazine, silver ion, copper ion

Abstract

This study aimed to develop Thai silk fibroin/gelatin (SF/GA) films incorporating various concentrations of silver sulfadiazine (SSD) and to investigate the effects of SSD and metallic ions (Ag(I) and Cu(II)) on chemical conformation of the SF/GA films. We found that the incorporation of SSD in the films changed the conformation of silk fibroin protein by increasing β-sheet content. The same phenomena was also observed with the other 2 metallic ions, Ag(I) and Cu(II), incorporated in the SF/GA films. This effect of metallic ions on the SF conformation transition in the SF/GA films may be similar to the phenomenon occurred during natural spinning process of the Bombyx mori silkworm. The SF/GA films incorporating SSD had therefore more stability, less water-insoluble fraction and extended degradation rate than the SF/GA films without SSD due to the higher content of stable β-sheet conformation. When cultured with L929 mouse fibroblast cells according to ISO 10993 part 5 standard, the SF/GA films incorporating SSD at all concentrations showed no cytotoxicity. The SSD released from the films also showed obvious anti-bacterial activity against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative) bacteria. We suggested that the SF/GA films incorporating SSD may be useful for some medical applications in which anti-bacterial effect was required.

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Author Biographies

Patchara Umprasert

Biomedical Engineering Program, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

Siriporn Damrongsakkul

Biomedical Engineering Research Center, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

Biomedical Engineering for Medical and Health Research Unit, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

Supansa Yodmuang

Biomedical Engineering Program, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

Biomedical Engineering Research Center, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

Biomedical Engineering for Medical and Health Research Unit, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

Juthamas Ratanavaraporn

Biomedical Engineering Program, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

Biomedical Engineering Research Center, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

Biomedical Engineering for Medical and Health Research Unit, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand

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Published In
Vol 25 No 5, May 31, 2021
How to Cite
[1]
P. . Umprasert, S. . Damrongsakkul, S. . Yodmuang, and J. Ratanavaraporn, “Effect of Silver Sulfadiazine and Metallic Ions on Properties of Thai Silk Fibroin/Gelatin Films for Anti-Bacterial Applications”, Eng. J., vol. 25, no. 5, pp. 13-20, May 2021.

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