Persistence of Nitrite-Oxidizing Bacteria in Entrapped Cell-Based Partial Nitrifying Reactor Treating Ammonia-Rich Wastewater

  • Pattaraporn Kunapongkiti Chulalongkorn University
  • Preeyaporn Pornkulwat Chulalongkorn University
  • Tawan Limpiyakorn Chulalongkorn University
  • Pratamaporn Homyok Chulalongkorn University
  • Panida Nayramitsattha Chulalongkorn University
  • Chaiwat Rongsayamanont Prince of Songkla University

Abstract

A phosphorylated-polyvinyl alcohol (PPVA) entrapped cell-based reactor was employed to promote partial nitrification for ammonia-rich wastewater treatment. High partial nitrification (66% of nitrite accumulation in average) was achieved along the 165 days of operation indicating that the majority of nitrite-oxidizing bacteria (NOB) activity was suppressed probably as a result of low oxygen environment created within the PPVA gel matrix. However, some portion of nitrate (5-17.6% of the influent ammonia) always appeared in the reactor throughout the operation period. Next-generation sequencing and clone library techniques revealed that NOB with different substrate affinities including Nitrobacter, Nitrospira lineage I and II existed within the gel matrix. The finding speculates that substrate gradient-like microenvironment within the gel matrix probably serves the different physiological groups of NOB to maintain their cells and activities in the reactor. Therefore, instead of using low oxygen environment in gel matrix as a sole control strategy, an additional strategy like promoting free ammonia inhibition in reactor is also needed to affirm the stability of long-term partial nitrification

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Author Biographies

Pattaraporn Kunapongkiti

Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand

Preeyaporn Pornkulwat

Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand

Tawan Limpiyakorn

1 Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand

2 Research Unit Control of Emerging Micropollutants in Environment, Chulalongkorn University, Bangkok 10330, Thailand

3 Research Network of Chulalongkorn University and National Nanotechnology Center (RNN), Thailand

Pratamaporn Homyok

Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand

Panida Nayramitsattha

Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand

Chaiwat Rongsayamanont

Environmental Assessment and Technology for Hazardous Waste Management Research Center, Faculty of Environmental Management, Prince of Songkla University, Songkhla 90110, Thailand

Research Program : The Development of Management System for Reduction and Control of Water  Contamination and Distribution in Songkhla Lake Basin and the Western Coastline of the South of Thailand, Center of Excellence on Hazardous Substance Management (HSM), Bangkok 10330, Thailand

Published In
Vol 23 No 2, Mar 31, 2019
How to Cite
[1]
P. Kunapongkiti, P. Pornkulwat, T. Limpiyakorn, P. Homyok, P. Nayramitsattha, and C. Rongsayamanont, “Persistence of Nitrite-Oxidizing Bacteria in Entrapped Cell-Based Partial Nitrifying Reactor Treating Ammonia-Rich Wastewater”, Eng. J., vol. 23, no. 2, pp. 1-10, Mar. 2019.