Simultaneous Hydrolysis and Fermentation of Sweet Sorghum Varieties (FS501 and KCS105) into Bioethanol Using <i>Saccharomyces steineri</i> – A Kinetics Study

  • Hanifrahmawan Sudibyo Universitas Gadjah Mada http://orcid.org/0000-0003-1905-129X
  • Jasman Jasman Universitas Nusa Cendana
  • Himawan Tri Bayu Murti Petrus Universitas Gadjah Mada
  • Agus Prasetya Universitas Gadjah Mada
  • Donny Widianto Universitas Gadjah Mada
  • Chusnul Hidayat Universitas Gadjah Mada
  • Satoshi Harashima Osaka University
  • Irfan Dwidya Prijambada Universitas Gadjah Mada

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Abstract

In this study, kinetics of bioethanol production by fermentation of three different substrates, which were artificial substrate and the juice of two sweet sorghum varieties (FS501 and KCS105) using Saccharomyces steineri, were examined using two proposed models by assuming that simultaneous hydrolysis and fermentation occurred. Fermentation of the substrate of FS501 and KCS105 juices showed better data fitting by using the modified version of the kinetics model while the fermentation of artificial substrate which was free of any other components followed Philippidis’s kinetics model. This difference was caused by the change of the yeast behavior in the form of the reduction of both the rate of fructose and/or glucose consumption by the yeast and the rate of fructose and or glucose conversion into ethanol during lag phase. As the consequence, sucrose hydrolysis seems very dominant in the FS501 and KCS105 juices fermentation during the lag phase. The change of behavior of the yeast was estimated being caused by the existence of “impurities” such as acetic acid, glycerol, nitrogen, phosphor, and potassium in the FS501 and KCS105 juices. From statistical analysis using correlation coefficient (between kinetics parameters and “impurities”), acetic acid was the most influential component to change the behavior.

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Author Biographies
Hanifrahmawan Sudibyo

Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia

Jasman Jasman

Science Education Department, Universitas Nusa Cendana, Kupang 85001 Indonesia

Himawan Tri Bayu Murti Petrus

Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia

Agus Prasetya

Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia

Donny Widianto

Faculty of Agriculture Technology, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia

Chusnul Hidayat

Faculty of Agriculture Technology, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia

Satoshi Harashima

Department of Biotechnology, Osaka University, Suita, Osaka 565-0871 Japan

Irfan Dwidya Prijambada

Faculty of Agriculture Technology, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia

Published
Vol 21 No 7, Dec 29, 2017
How to Cite
H. Sudibyo, “Simultaneous Hydrolysis and Fermentation of Sweet Sorghum Varieties (FS501 and KCS105) into Bioethanol Using <i>Saccharomyces steineri</i&gt; – A Kinetics Study”, Engineering Journal (Eng. J.), vol. 21, no. 7, pp. 105-121, Dec. 2017.

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